Table 1.
Thermal stability of class I and class II MHC molecules at neutral and mildly acidic pH
| Molecule | Peptide | pH | Tm, °C |
|---|---|---|---|
| I-Ek | — | 7.0 | 62 ± 1 |
| I-Ek | ANERADLIAYLKQATK | 7.0 | 70 ± 1 |
| I-Ek | — | 5.0 | 63 ± 1 |
| I-Ek | ANERADLIAYLKQATK | 5.0 | 68 ± 1 |
| I-Ad | ASFEAQGALANIAVDKA | 7.0 | 62 ± 1 |
| I-Ad | ASFEAQGALANIAVDKA | 5.0 | 61 ± 1 |
| HLA-A2 | ILKEQVHGV | 7.0 | 59 ± 1 |
| HLA-A2 | ILKEQVHGV | 5.5 | 45 ± 1 |
| HLA-B27 | Mixed | 7.0 | 66 ± 1 |
| HLA-B27 | Mixed | 7.0† | 63 ± 2 |
| HLA-B27 | Mixed | 5.0† | 52 ± 2 |
| Kd* | — | 7.0 | 45 ± 1 |
| Kd* | TYQRTRALV | 7.0 | 57 ± 1 |
| Kd | AYISSGSSTL | 7.0 | 54 ± 1 |
| Kd | AYISSGSSTL | 6.0 | 54 ± 1 |
| Kd | AYISSGSSTL | 5.5 | 54 ± 1 |
*
From ref. 9.
†
Measured in the presence of 300 mM urea.