Table 2.
Transdominant repression of DRACAT expression by CIITA mutants in RJ2.2.5
| Parameters | 1 | 2 | 3 |
|---|---|---|---|
| pDRA300CAT | + | + | + |
| FLAG.CIITA | + | + | + |
| Control/mutant | pcDNA3 | CIITA (Δ132–301) | CIITA-GTP2 (ΔDAYG) |
| % inhibition* | — | 94.5 | 97.2 |
*
Wild-type FLAG.CIITA8 was cotransfected with pDRA300CAT in the presence of control, pcDNA3, or different mutant forms of CIITA. The subsequent CIITA activity of each sample was assessed and the transdominant-negative function of the mutant constructs was calculated as (average of three experiments):
 |