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. 2003 Oct;47(10):3326–3331. doi: 10.1128/AAC.47.10.3326-3331.2003

FIG. 2.

FIG. 2.

ere(A)pIP1100 cassette sequence and features (A) and comparison of the ere(A) attC sites to the short attC sites of other resistance cassettes (B). (A) The EreA EM esterase amino acid sequence is shown underneath the coding part of the cassette. The potential ribosome binding site is shown in bold, the proposed start codon in the former characterization of ere(A) is shown by a grey box (an additional A upstream of the overlined AA put this ATG in frame with the 3′ end of the gene), and the sequences that are complementary in the CS and ICS of the circularized ere(A) cassette are underlined. (B) CLUSTAL X (1.8) multiple sequence alignment of the attC sites from the following cassettes: aadA5 (GenBank no. AF137361), aadA1b (GenBank no. M95287), orfD (GenBank no. M95287), aadA1a (GenBank no. X12870), aadA3 (GenBank no. AF047479), aadA6 (GenBank no. AF140629), qacF (GenBank no. AF034958), aacAIIa (GenBank no. M29695), catB3 (GenBank no. U13880), catB5 (GenBank no. X82455), aadA7 (GenBank no. AF224733), sat (GenBank no. X15995), aadB (GenBank no. L06418), aadA2 (GenBank no. X68227), dfrB1 (GenBank no. U36276), dfrB3 (GenBank no. X72585), dfrB2 (GenBank no. J01773), ere(A)pLQ [ere(A) of pLQ1723; GenBank no. AF099140], ere(A)pIP [ere(A) of pIP1100; GenBank no. AY183453]. Putative IntI1 binding domains as defined by Stokes et al. (25) are boxed; conserved positions are indicated by stars.