TABLE 1.
Bacterial strains and relevant plasmids
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| E. coli | ||
| DH5α | supE44ΔlacU169 (φ80lacZ′ ΔM15) ΔargF hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | Laboratory collection |
| UB1637 | his lys trp recA56 rpsL | 13 |
| UB5201 | pro met recA56 gyrA | 13 |
| BM2195 | Wild-type, pIP1100, pIP1101, pIP1102 | 1 |
| ω165 | UB1637 R388 p2187 p112 | This work |
| ω171 | UB1637 R388 p2194 pTRC99A | This work |
| ω172 | UB1637 R388 p2194 p112 | This work |
| ω173 | UB1637 R388 p2195 pTRC99A | This work |
| ω174 | UB1637 R388 p2195 p112 | This work |
| P. stuartii | Wild-type, pLQ1723 [ere(A)] | P. H. Roy |
| Plasmids | ||
| R388 | Tpr Sur Tra+ IncW | 27 |
| pSU38 | Kanr oriP15A | 4 |
| pTRC99A | Apr oriColE1, expression vector | Pharmacia |
| p112 | pTRC99A::int11 | 20 |
| p2247 | 1,298 bp EcoRI-BamHI PCR fragment (ereA1 and ereA2) from pLQ1723 in pSU38, ere(A)pLQ1723 L101S, Kanr | This work (AY183454) |
| p1792 | 5.4-kb PCR fragment (I2DW and orfX-Tn7) from pIP1100 in pCR2.1, Kanr Apr Emr | This work (AY183453) |
| p1818 | 1,290-bp SacI-BamHI PCR fragment (ereAStQ.L2 and ereA2) from p1792 in pSU38, ere(A)pIP1100, Kanr Emr | This work |
| p1988 | 1,298-bp EcoRI-BamHI PCR fragment (ereA1 and ereA2) from pLQ1723 in pSU38, ere(A)pLQ1723, Kanr Emr | This work |
| p2187 | 1,492-bp BamHI PCR fragment (SatEnd and aadA1) from p1792 in pSU38, lacZα orientation, Kanr Emr | This work |
| p2188 | 1,492-bp BamHI PCR fragment (SatEnd and aadA1) from p1792 in pSU38, anti-lacZα orientation, Kanr Emr | This work |
| p2194 | EcoRI deletion in p2187, ere(A)(-aadA1) attC site, Kanr | This work |
| p2195 | PstI deletion in p2187, sat(-ere(A)) attC site, Kanr | This work |