TABLE 2.
Time of growth on Fe(II) medium (h) | pH | % of initial As concn
|
||
---|---|---|---|---|
As | As(III) | As(V) | ||
0 | 2.4 | 100 | 81 | 9 |
24 | 2.57 | 96 | 64 | 0 |
40 | 2.48 | 94 | 64 | 0 |
48 | 2.4 | 83 | 73 | 0 |
72 | 2.35 | 85 | 74 | 2 |
Strain CC1 was grown on Fe(II) medium. Samples were removed throughout growth, treated with chloroform to kill the cells, supplemented with As(III), shaken at room temperature for 5 min, and frozen until analysis. The pH and the concentrations of soluble As, As(III), and As(V) were determined and expressed as a percentage of the soluble arsenic concentration at the beginning of the experiment.