Table 1.
Oligonucleotides
| Name* | Sequence (5′ → 3′)† | Source‡ |
|---|---|---|
| AmplifFINDER anchor | cacgaattcaCTATCGATTCTGGAACCTTCAGAGG | CLONTECH |
| Anchor primer | ctggttcggcccaCCTCTGAAGGTTCCAGAATCGATAG | CLONTECH |
| CCC32 (SK primer) | TCTAGAACTAGTGGATC | LI |
| CCC34 (T3 primer) | AATAACCCTCACTAAAG | LI |
| CCC35 (T7 primer) | AATACGACTCACTATAG | LI |
| CCC52 (Fig1 524–542) | cggaattcggtaccGGAGAAGATGCCAGAAAAG | Paragon |
| CCC53 (Fig1ps 1165–1149) | cggaattcggtacCACAAGACTCTTGGGGG | Paragon |
| CCC54 (Fig1 790–773) | cggaattcggtacCGTAAGGCTTCTGCAAAG | Paragon |
| CCC55 (Fig1 610–588) | cgtgatcaagcTTGAGTGCCATCTGGTAGATGTC | Bio-Synthesis |
| CCC56 | CCTCTGAAGGTTCCAGAATCGATAG | Bio-Synthesis |
| CCC57 (SP6 primer) | ATTTAGGTGACACTATAG | Bio-Synthesis |
| CCC58 (Fig1 410–393) | GTGAGAGCTGGGCATTCG | Bio-Synthesis |
| CHU3 (Fig1ps 2213–2194) | CCTCGGACATCACATCTCCC | Paragon |
| CHU39 (Fig1ps 1711–1730) | GGGCCAGTAGAGGTGGGACT | Paragon |
| CHU44 (Fig1ps 811–789) | AGGAGTCGGGGTGGGGATAGGGG | Paragon |
| λgt11 forward primer | GGTGGCGACGACTCCTGGAGCCCG | NEB#1218 |
| λgt11 reverse primer | TTGACACCAGACCAACTGGTAATG | NEB#1222 |
*
Oligonucleotides complementary to Fig1 or partially spliced Fig1 (Fig1ps) have sequence coordinates shown.
†
Lowercase sequences indicate added restriction site tails for CCC52, CCC53, CCC54, and CCC55. AmpliFINDER anchor and anchor primer lowercase letters indicate sequences not complementary to each other.
‡
Oligonucleotides were synthesized in the Laboratory of Immunology (LI) as described (5) or purchased from CLONTECH, Paragon Biotechnology, Bio-Synthesis (Lewisville, TX), or New England Biolabs.