TABLE 2.
Sensitivity of purified enterocin RJ-11 to various proteases and heat treatment
| Treatmenta | Residual activity (AU/ml)b |
|---|---|
| Control | 64,000 |
| Protease (pH 7.5, 37°C)c | 0 |
| Proteinase K (pH 7.5, 37°C) | 0 |
| α-Chymotrypsin (pH 7.5, 25°C) | 8,000 |
| Trypsin (pH 7.5, 25°C) | 32,000 |
| Papain (pH 6.0, 37°C) | 64,000 |
| Pepsin (pH 2.0, 37°C) | 32,000 |
| Control | 102,400 |
| 100°C, 15 min | 102,400 |
| 100°C, 30 min | 102,400 |
| pH 2, 30°C, 6 h | 102,400 |
| pH 12, 30°C, 6 h | 102,400 |
a
In each protease treatment,the sample was incubated for 7 h under the optimum pH and temperature condition indicated in parenthesis.
b
L. monocytogenes was used as the indicator strain.
c
A product of S. griseus.