TABLE 2.

Effect of GSH on lag phases upon subcultivation of different strains of L. lactis^a

Strain	Ability to accumulate GSH when grown anaerobically	Lag phase (h) with the following GSH concn in preculture
		0	0.2 mM
L. lactis subsp. cremoris strains
SK11	+	12.60 ± 0.60	6.67 ± 0.12
MG1363	−	6.17 ± 0.17	6.42 ± 0.17
N1ZO B30	+	6.79 ± 0.39	5.38 ± 0.11
NIZO B33	+	11.77 ± 0.34	5.55 ± 0.35
NIZO B42	+	12.67 ± 0.19	6.35 ± 0.23
NIZO B67	−	10.45 ± 0.22	10.79 ± 0.33
NIZO B74	+	5.94 ± 0.28	4.15 ± 0.14
L. lactis subsp. lactis strains
IL-1403	−	8.93 ± 0.32	9.35 ± 0.34
NIZO B10	−	5.56 ± 0.08	5.60 ± 0.04
NIZO B11	−	3.46 ± 0.11	3.79 ± 0.11
NIZO B13	−	2.79 ± 0.11	2.85 ± 0.04
NIZO B20	−	7.99 ± 0.34	8.01 ± 0.16
L. lactis subsp. lactis biovar diacetylactis strains
NIZO B81	−	7.15 ± 0.08	7.32 ± 0.13
NIZO B86	−	9.50 ± 0.30	9.67 ± 0.14
NIZO B90	−	4.00 ± 0.26	4.04 ± 0.09
NIZO B93	+	7.31 ± 0.26	6.15 ± 0.14

^aCells were precultured in CDM lacking GSH or supplemented with 0.2 mM GSH and then treated with 5 mM H₂O₂ for 5 min. The cells were pelleted after the H₂O₂ treatment and washed once with saline, and then they were resuspended in the same volume of CDM and inoculated for growth experiments as described in Materials and Methods.