TABLE 1.

Oligonucleotides used in this study (excluding those used for DNA sequencing)

Oligonucleotide	Nucleotide sequence (5′ to 3′)a	Amplified product
5rev1	GTTCAATTGACGGATATG	mlp repeat region probe
5for5	CACCAGCACCAGTAATC	mlp repeat region probe
pUC-Bam	CTTGGATCCCTGCAGGTCGACTCTAG	3′ end of mlp and downstream
Mlp-Paw-C	AAGGATCCCATGGAAGACCAAGAAGCT	3′ end of mlp and downstream
Mlp-C-check	GCTATGAATCTGTTAAGCCAC	3′ end of mlp and downstream
BspA-Xba	CGTTTCTAGAACTTGTTAGTAATGCCGG	bspA
BspA-LPX-Pst-His	AACTGCAGAGTGATGATGGTGATGATGTTCT CCTGTTGATGGTAATCCTCCTTCTGTAATATCCGCACC	bspA
Term-Xho	TAACTCGAGTTTTGCAGTTCATTCGTTAG	bspA terminator
Term-Hind	AAGGAAGCTTTGGTAATGGGGATTGCC	bspA terminator
BspA-Eco-stop	CCGAATTCTAACGAATGAACTGC	bspA
BspA-Eco-SS	AAGAATTCTAGCTTCGATGACAATGG	bspA 5′ end
BspA-SS-Pst-His	AACTGCAGAGTGATGATGGTGATGATGTACA TCATCAGATGCCGCATGAATACT	bspA 5′ end
Rlp′′-Pst-Xho	AGTCTGCAGCTCGAGCAGATGCAATTAAGAAT	rlp 3′ end
Rlp-Sal-Apa	TTTGGGCCCGTCGACTCCTCTTAATTCAATCGC	rlp 3′ end
MlpN-Xba	AAGTCTAGATAAGAAACGTAAGGCTG	mlp 5′ end
MlpN-Xho-His	TTACTCGAGTTAGTGATGATGGTGATGATGAACACTACCAAACGTGAC	mlp 5′ end
Mlprep-Xba	CATTCTAGATGACCCAACTGGCAA	mlp internal fragment
Mlprep-Pst-His	GTACTGCAGAGTGATGATGGTGATGATGATT ATCAATAGTTGCAGT	mlp internal fragment
CFTR-5′-Pst	AAACTGCAGGATCATATGATCCAGATAATAA GGAAGAACGTGGAGGAAGTTACGACCCGG ATAACAAGGAAGAACGTGGAGGA	CFTR, peptide-encoding region
CFTR-3′-Xho	AAACTCGAGAACGTTCTTCCTTATTATCTGGA TCATATGATCCTCCACGTTCTTCCTTGTTATCCGGGTCGTAACT	CFTR, peptide-encoding region

^aUnderlining indicates restriction enzyme cleavage sites.