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Detection of donor-derived cells in the primary recipients. Five hundred Lin−/CD71−/class Ihigh/c-kitlow cells or Lin−/CD71−/class Ihigh/c-kit<low cells from 5-FU-treated B6 Ly5.1 mice were transplanted into irradiated B6 Ly5.2 recipients along with 2 × 105 compromised B6 Ly5.2 cells. Six months later, cells from various organs were stained with a panel of mAbs and donor-specific anti-Ly5.1 mAb.
