Figure 4.

Detection of donor-derived cells in a tertiary recipient from a secondary recipient originally reconstituted with Lin⁻/CD71⁻/class I^high/c-kit^<low cells. Six months after the secondary transplantation, BMCs (1 × 10⁶) from the secondary recipients originally reconstituted with either Lin⁻/CD71⁻/class I^high/c-kit^low or ^Lin-/CD71⁻/class I^high/c-kit^<low cells were further retransplanted into the irradiated B6 Ly5.2 tertiary recipients. All tertiary recipients originally reconstituted with the former (c-kit^low) cells died within 10 days whereas all tertiary recipients (five mice) reconstituted with the latter cells survived more than 6 months; one mouse was killed 1 month after the transplantation, and the various hematolymphoid organs were examined for the phenotypes of hematolymphoid cells.