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Northern analysis of Tri16 and Tri1 transcript accumulation by F. sporotrichioides strains grown in liquid culture. Total RNA was isolated for each strain from mycelia collected from cultures grown for 23, 27, and 36 h in YEPD-5G medium. Strains are (left to right) the wild type (NRRL 3299), ΔTri16 transformants (Tx #4, Tx #7, and Tx #20), and a transformant (Tx #22) resulting from ectopic integration of pKO16. rRNA bands from the agarose gel stained with ethidium bromide are shown.
