TABLE 1.
Bacterial strains, plasmids, and PCR primers used in this study
| Strain, plasmid, or primer | Relevant characteristic(s) or sequence | Source or reference |
|---|---|---|
| V. fischeri | ||
| ES114 | Wild-type light-organ isolate from E. scolopes | 5 |
| ESR1 | Spontaneous Rfr mutant of ES114 | 14 |
| JRM100 to JRM104 | Five ES114 clones with mini-Tn7 insertion; Emr | This study |
| JRM200 | ES114 clone with mini-Tn7 insertion; Cmr | This study |
| E. coli | ||
| BW23474 | Δlac-169 robA1 creC510 hsdR514 uidA(ΔMluI)::pir116 endA(BT33) recA1 | 28 |
| CC118λpir | Δ(ara-leu) araD ΔlacX74 galE galK phoA20 thi-I rpsE rpoB argE(Am) recA1; lysogenized with λpir | 16 |
| Plasmids | ||
| pEVS94S | R6K ori oriT Emr | 41 |
| pEVS104 | Derivative of pRK2013; conjugal tra and trb genes | 41 |
| pEVS107 | pEVS94S derivative, mini-Tn7; mob; Emr Knr | This study |
| pUXBF13 | R6K ori; tns genes; Apr | 4 |
| pCR2.1 | PCR product cloning vector; ColE1 ori; Apr Knr | Invitrogen Inc. |
| pJRM3 | pEVS107 with Cmr replacing Emr | This study |
| pEVS35 | CmrXbaI fragment cloned into XbaI site of pBC | 39 |
| Primers | ||
| JM1 | 5′ GTTACACGTTACTAAAGGG 3′ | This study |
| JM2 | 5′ ACCAGACCGTTCAGCTGG 3′ | This study |
| JM3 | 5′ GGAACATGTGTGGTATGG 3′ | This study |
| JM4 | 5′ GACAGTCATCTATTCAAC 3′ | This study |