V. fischeri |
|
|
ES114 |
Wild-type light-organ isolate from E. scolopes
|
5 |
ESR1 |
Spontaneous Rfr mutant of ES114 |
14 |
JRM100 to JRM104 |
Five ES114 clones with mini-Tn7 insertion; Emr
|
This study |
JRM200 |
ES114 clone with mini-Tn7 insertion; Cmr
|
This study |
E. coli |
|
|
BW23474 |
Δlac-169 robA1 creC510 hsdR514 uidA(ΔMluI)::pir116 endA(BT33) recA1
|
28 |
CC118λpir
|
Δ(ara-leu) araD ΔlacX74 galE galK phoA20 thi-I rpsE rpoB argE(Am) recA1; lysogenized with λpir
|
16 |
Plasmids |
|
|
pEVS94S |
R6K ori oriT Emr
|
41 |
pEVS104 |
Derivative of pRK2013; conjugal tra and trb genes |
41 |
pEVS107 |
pEVS94S derivative, mini-Tn7; mob; Emr Knr
|
This study |
pUXBF13 |
R6K ori; tns genes; Apr
|
4 |
pCR2.1 |
PCR product cloning vector; ColE1 ori; Apr Knr
|
Invitrogen Inc. |
pJRM3 |
pEVS107 with Cmr replacing Emr
|
This study |
pEVS35 |
CmrXbaI fragment cloned into XbaI site of pBC |
39 |
Primers |
|
|
JM1 |
5′ GTTACACGTTACTAAAGGG 3′ |
This study |
JM2 |
5′ ACCAGACCGTTCAGCTGG 3′ |
This study |
JM3 |
5′ GGAACATGTGTGGTATGG 3′ |
This study |
JM4 |
5′ GACAGTCATCTATTCAAC 3′ |
This study |