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. 2003 Oct;69(10):6007–6017. doi: 10.1128/AEM.69.10.6007-6017.2003

TABLE 1.

Enumeration of microbial cells in the different gut sections of C. ugandensisa

Gut section Vol (μl) No. of cellsb (106 cells per gut section)
Cocci Short rods Long rods Filaments Spirochetes Total
Crop ND 0.5 ± 0.2 0.4 ± 0.0 0.1 ± 0.0 1.0 ± 0.2
Midgut 0.1 ± 0.0 0.4 ± 0.0 4.8 ± 0.7 2.0 ± 0.2 0.1 ± 0.1 0.1 ± 0.0 7.5 ± 0.5
P1 0.8 ± 0.2 3.6 ± 1.5 16.4 ± 1.7 1.7 ± 0.5 0.1 ± 0.0 0.9 ± 0.2 22.7 ± 3.9
P3 0.5 ± 0.2 4.8 ± 0.6 28.1 ± 0.8 2.4 ± 0.5 0.3 ± 0.1 4.3 ± 0.7 39.9 ± 1.2
P4 0.1 ± 0.0 2.1 ± 0.2 7.5 ± 0.2 0.6 ± 0.1 0.1 ± 0.1 0.6 ± 0.1 11.0 ± 0.7
P5 0.2 ± 0.1 1.1 ± 0.4 4.5 ± 0.2 0.1 ± 0.0 0.1 ± 0.0 6.2 ± 0.2
Total gutc 1.7 ± 0.3 12.6 ± 1.7 61.8 ± 3.5 6.9 ± 1.0 0.7 ± 0.1 6.1 ± 0.4 88.3 ± 6.2
a

Enumeration was done by the DAPI staining technique on polycarbonate filters, differentiated by cell morphotypes (short rods, <5 μm; long rods, 5 to 15 μm; filament, >15 μm). Values are means (± mean deviation) of two independently prepared homogenates. The volumes of the gut sections were estimated by approximation to different geometrical forms (69). Volume estimation values are averages (± standard deviation) of 15 freshly dissected termite guts.

b

Total, sum of all cells in a section. ND, not determined. —, below the detection limit (<0.1 × 106 cells per gut section).

c

Sum of cells of a morphotype in all sections.