TABLE 1.
Gut section | Vol (μl) | No. of cellsb (106 cells per gut section)
|
|||||
---|---|---|---|---|---|---|---|
Cocci | Short rods | Long rods | Filaments | Spirochetes | Total | ||
Crop | ND | 0.5 ± 0.2 | 0.4 ± 0.0 | 0.1 ± 0.0 | — | — | 1.0 ± 0.2 |
Midgut | 0.1 ± 0.0 | 0.4 ± 0.0 | 4.8 ± 0.7 | 2.0 ± 0.2 | 0.1 ± 0.1 | 0.1 ± 0.0 | 7.5 ± 0.5 |
P1 | 0.8 ± 0.2 | 3.6 ± 1.5 | 16.4 ± 1.7 | 1.7 ± 0.5 | 0.1 ± 0.0 | 0.9 ± 0.2 | 22.7 ± 3.9 |
P3 | 0.5 ± 0.2 | 4.8 ± 0.6 | 28.1 ± 0.8 | 2.4 ± 0.5 | 0.3 ± 0.1 | 4.3 ± 0.7 | 39.9 ± 1.2 |
P4 | 0.1 ± 0.0 | 2.1 ± 0.2 | 7.5 ± 0.2 | 0.6 ± 0.1 | 0.1 ± 0.1 | 0.6 ± 0.1 | 11.0 ± 0.7 |
P5 | 0.2 ± 0.1 | 1.1 ± 0.4 | 4.5 ± 0.2 | 0.1 ± 0.0 | — | 0.1 ± 0.0 | 6.2 ± 0.2 |
Total gutc | 1.7 ± 0.3 | 12.6 ± 1.7 | 61.8 ± 3.5 | 6.9 ± 1.0 | 0.7 ± 0.1 | 6.1 ± 0.4 | 88.3 ± 6.2 |
Enumeration was done by the DAPI staining technique on polycarbonate filters, differentiated by cell morphotypes (short rods, <5 μm; long rods, 5 to 15 μm; filament, >15 μm). Values are means (± mean deviation) of two independently prepared homogenates. The volumes of the gut sections were estimated by approximation to different geometrical forms (69). Volume estimation values are averages (± standard deviation) of 15 freshly dissected termite guts.
Total, sum of all cells in a section. ND, not determined. —, below the detection limit (<0.1 × 106 cells per gut section).
Sum of cells of a morphotype in all sections.