TABLE 2.
Cell densities in different gut segments of C. ugandensis and proportion of cells hybridizing with domain-specific FISH probes relative to the number of DAPI-stained cells in homogenates of the respective sectionsd
| Gut section | Cell densitya (109 cells ml−1) | Bacteriab (%) | Archaeac (%) |
|---|---|---|---|
| Midgut | 82.5 ± 27.9 | 63.7 ± 12.1 | — |
| P1 | 22.9 ± 1.5 | 24.7 ± 5.9 | — |
| P3 | 54.4 ± 13.4 | 29.9 ± 9.8 | 1.6 ± 1.0 |
| P4 | 85.9 ± 37.7 | 39.6 ± 13.7 | 3.8 ± 2.8 |
a
Total cell counts (DAPI) per gut segment were converted to cell densities by using the average volume of the respective segment (Table 1).
b
Mixture of probes EUB338, EUB338-II, and EUB338-III (35% formamide) (20).
c
Probe ARCH915 (35% formamide) (66). —, below the detection limit (<0.1%).
d
Values are means ± mean deviations of two independently prepared homogenates.