. 2003 Oct;69(10):6007–6017. doi: 10.1128/AEM.69.10.6007-6017.2003

TABLE 3.

Abundance of bacterial cells in different gut sections of C. ugandensis hybridizing with various group-specific oligonucleotide probes, analyzed by FISH

Gut section	Relative abundance (% EUB) ± SD^m
	Proteobacteria				CFB phylum^e	Planctomycetes^f	HGC bacteria^g	LGC bacteria			Total^k
	α^a	β^b	γ^c	δ^d	CFB phylum^e	Planctomycetes^f	HGC bacteria^g	LGC354^h	P1 clusterⁱ	Sp. cluster^j	Total^k
Midgut	1.6 ± 0.7	—^l	—	3.2 ± 1.5	1.8 ± 0.1	3.9 ± 0.8	—	2.2 ± 0.2	1.4 ± 0.6	0.7 ± 0.5	14.8
P1	—	—	—	—	—	9.3 ± 8.1	—	0.4 ± 0.3	4.4 ± 1.9	0.6 ± 0.5	14.7
P3	0.2 ± 0.0	1.3 ± 1.5	1.0 ± 1.0	2.0 ± 0.6	3.1 ± 1.8	36.0 ± 9.9	—	0.3 ± 0.1	0.3 ± 0.1	12.6 ± 4.9	56.8
P4	3.5 ± 0.9	4.3 ± 2.1	3.1 ± 1.7	2.8 ± 0.8	12.3 ± 6.0	9.9 ± 3.9	4.1 ± 1.2	3.0 ± 0.0	0.8 ± 0.5	3.8 ± 1.8	47.6

Mixture of probes ALF1b (46) and ALF968 (50) (35% formamide).

Probe BET42a (35% formamide) (46), used together with the unlabeled competitor cGAM42a.

Probe GAM42a (35% formamide) (46), used together with the unlabeled competitor cBET42a.

Probe SRB385 (30% formamide) (2).

Mixture of probes CF319a and BAC303 (35% formamide) (45). CFB, Cytophaga-Flexibacter-Bacteroides.

Mixture of probes PLA866 and PLA46 (30% formamide) (51) and unlabeled competitor cPLA886.

Probe HGC69a (30% formamide) (62) for gram-positive bacteria with high G + C content.

Mixture of probes LGC354A, LGC354B, and LGC354C (35% formamide) (48) for the Bacillales and Lactobacillales.

ⁱ

Probe P1C95 (35% formamide) for the P1 cluster (this study), used together with the helper probe HelperP1C95.

Probes T125 and T433 (35% formamide) for the Sporobacter cluster (this study), used together with the helper probes HelperT125 and HelperT433.

Sum of the results obtained with individual probes.

—, below the detection limit (<0.1%).

Values are based on those obtained with the EUB probe mixture (see Table 2) and represent means ± standard deviations of two independently prepared homogenates.