TABLE 3.

Functionality of arbitrary PCR in different Tn917 mutants

Mutant	Functionalitya of PCR with first arbitrary primer:
	STAPHarb1 and first/second Tn917 primers:				STAPHarb2 and first/second Tn917 primers:
	917-5.3/917-5.2	917-5.3/917-5.1	917-5.2/917-5.1	917-3.3/917-3.2	917-5.3/917-5.2	917-5.3/917-5.1	917-5.2/917-5.1	917-3.3/917-3.2
M10	+	+	+	+	+	+	+	+
M11	−	−	−	+	−	+	−	+
M12	+	+	+	−	−	−	−	−
M15	+	+	+	+	−	−	+	+
M16	+	−	+	+	+	+	+	−
M20	−	−	+	+	+	+	+	−
M21	−	−	−	+	−	+	+	+
M22	+	−	−	+	+	+	−	+
M24	−	+	+	+	+	+	+	+

^a+, Arbitrary PCR fragments containing Tn917 flanking chromosomal DNA.