TABLE 2.
Compositions of Yersinia selective media
| Chemical agent | Concn (g/liter) in:
|
||
|---|---|---|---|
| BIN agara | CIN agarb | MacConkey agarb | |
| Bacto Proteose Peptone | 10d | 3c | 3 |
| Calf brains, infusion from | 200d | ||
| Beef heart, infusion from | 250d | ||
| Bacto Peptone | 17c | 17 | |
| Bacto Yeast Extract | 2c | ||
| Bacto Agar | 15d | 13.5c | 13.5 |
| Mannitol | 20c | ||
| Lactose | 10 | ||
| Dextrose | 2d | ||
| Neutral red | 0.03 | 0.03 | |
| Sodium pyruvate | 2c | ||
| Magnesium sulfate heptahydrate | 0.01c | ||
| Disodium phosphate | 2.5d | ||
| Sodium chloride | 5d | 1c | 5 |
| Sodium cholate | 0.5 | 0.5 | |
| Sodium deoxycholate | 0.5 | 0.5 | |
| Bacto Bile Salts, no. 3 | 1.5 | ||
| Crystal violet | 0.001 | 0.001 | 0.001 |
| Irgasan | 0.0008 | 0.004 | |
| Cefsulodin | 0.004 | ||
| Novobiocin | 0.0025 | ||
| Nystatin | 0.025 | ||
a
Developed by the Israel Institute for Biological Research.
b
According to Difco.
c
Component in basal CIN medium.
d
Component included in the BHIA (Difco), which is added at 52 g/liter. (Growth performance differences between BHIA batches may occur. Batch no. 0418-17-6 provided good growth.)