Table 1.
Lack of restriction of TCR β chain sequences from insulin B-(9–23)-reactive clones
| Clone | Designation | Regions
|
|||
|---|---|---|---|---|---|
| Vβ | nDn | Jβ | |||
| 12-4.1 | Vβ2 | Jβ2.6 | LYCTCS | P G L G K | EQY |
| 12-4.4 | Vβ12 | Jβ1.3 | YLCASS | P G Q G T | TLY |
| 4-7.2 | Vβ1 | Jβ1.3 | YFCASS | Q S R T | GNT |
| 6-4.3 | Vβ2 | Jβ1.1 | LYCTCS | A A G G G | TEV |
| 8-1.3 (LN) | Vβ6 | Jβ1.6 | FLCAS | T S G T G Q G | SPL |
| 12-3.20 | Vβ14 | Jβ2.5 | YLCAWS | R L G G | NQD |
| 8-1.9 (LN) | Vβ4 | Jβ1.1 | YFCASS | P D N A | NTE |
| 8-1.1 (LN) | Vβ12 | Jβ2.6 | YLCASS | L G W G D | EQY |
| 12-1.19 | Vβ6 | Jβ1.1 | FLCASS | I L G Q | NTE |
| 12-2.35 | Vβ8.3 | Jβ1.6 | YFCASS | P S G R | NSP |
| 6-6.4 | Vβ10 | Jβ2.5 | YLCASS | W G Q G G | DTQ |
The partial amino acid sequences of the TCR β chains include the terminal portion of the Vβ region, the nDn region (underlined), and the proximal portion or the Jβ region. All of the clones react to insulin peptide B-(9–23). The majority of clones were isolated from spontaneous islet lesions of female NOD mice. Clones with the suffix (LN) were isolated from cervical lymph nodes after nasal immunization with the B-(9–23) peptide. This panel represents clones from eight different mice ranging from 4 to 12 weeks of age (first number of the clone indicates the age of the mouse at the time of T cell isolation).