TABLE 2.
Primers used in PCR experiments
| Primer | Sequence (5′→3′)a | Nucleotide position | EMBL database accession no. |
|---|---|---|---|
| glyc-g1 | CGGTCAACAAAGAAGCGATTCCG (F) | 2144349-2144372 | AL935263 |
| Lpupp-h1 | TATCCATCGCCATGATGGCTGAACC (R) | 698-722 | AJ012720 |
| Lp-IS30b | GGTAGATTGYAAAWTTAATCCGA | 1219-1241 | AF459445 |
| 2239-2261 | AF459445 | ||
| LpIS1077F | GCCTTTAATAAGCAAACTCGC (F) | 553-573 | AF459445 |
| LpIS30in | GATTAAAGCGTTCATTACTACC (R) | 2062-2083 | AF459445 |
| LpIS1077F | GCCTTTAATAAGCAAACTCGC (F) | 553-573 | AF459445 |
| ybher1 | GAAGTAGCCAAAGAGCACGG (R) | 2625-2644 | AF459445 |
| Lp-IS30d | GGTTATGCTGACAAATTTGTCC (R) | 2211-2232 | AF459445 |
| M13Rev | CAGGAAACAGCTATGACCATGATTAC (F) | 456-481 | X02514 |
a
F, forward primer; R, reverse primer.
b
This primer binds to both ends of ISLp11. Y = C or T; W = A or T.