TABLE 1.
Microbiological materials
| Material | Relevant trait(s) | Source or reference |
|---|---|---|
| E. coli strains | ||
| VCS257 | DP50 derivative | Stratagene |
| DH5α | Cloning strain, recA1 endA1 gyrA96 thi1 relA1 hsdR17 supE44 lac | Gibco-BRL |
| BL21(DE3) | Expression host for pET vectors | Novagene |
| Plasmids and cosmids | ||
| pBSK+ | pBluescriptSK+ multicopy cloning vector, Ampr | Stratagene |
| pTZ19R | High-copy-number cloning vector, Cmr | Amersham, Pharmacia |
| pWE15 | Cosmid cloning vector for construction of DNA libraries, Ampr | Stratagene |
| pIIIE5 | pWE15 containing a 20.7-kb insert with possible agarase genes from microbial consortium | This study |
| pXE1 | pWE15 containing a 25-kb insert with possible lipase genes from microbial consortium | This study |
| pVIIC10 | pWE15 containing a 34.7-kb insert with possible agarase genes from microbial consortium | This study |
| pXB11 | pWE15 containing a ca. 20-kb insert with possible agarase genes from microbial consortium | This study |
| pXIVA3 | pWE15 containing a 38.1-kb insert with possible agarase genes from microbial consortium | This study |
| pIIIF2 | pWE15 containing a 28-kb insert with potential pectate lyase genes from microbial consortium | This study |
| pAGUB | pET19B (Novagene) carrying the aguB gene in the NdeI site | This study |