TABLE 2.
ORFs and genes identified for the sequenced contigs of cosmid clones pVIIC10, pXIVA3, pIIIE5, pXB11, pXE1, and pIIIF2a
| Cosmid | ORF or gene | Possible function | |
|---|---|---|---|
| pXE1 | aprE | AprE | |
| aprF | AprF | ||
| lipA | Hypothetical protein | ||
| lipB | Lipase | ||
| uvs005 | Hypothetical protein | ||
| uvs006 | Possible DNA helicase | ||
| uvs007 | Hypothetical protein | ||
| uvs008 | Possible sensor or response regulator | ||
| uvs009 | Possible sensor or response regulator | ||
| uvs010 | Possible Na+/H+ antiporter | ||
| uvs011 | Putative aminotransferase | ||
| uvs012 | Hypothetical protein | ||
| uvs013 | Hypothetical protein | ||
| aprD | AprD | ||
| uvs015 | Cytochrome c biogenesis protein CcmG | ||
| uvs016 | Cytochrome c biogenesis protein CycK | ||
| uvs017 | β-Lactamase | ||
| amiA | Enantiomer-selective amidase | ||
| pVIIC10 | uvs031 | Transporter protein | |
| uvs032 | RNA polymerase, σH | ||
| uvs033 | Peptidoglycan binding protein | ||
| aguA | β-Agarase B | ||
| aguB | β-Agarase I | ||
| uvs036 | Hypothetical protein | ||
| uvs037 | Hypothetical protein | ||
| uvs038 | Membrane protein | ||
| uvs039 | Hypothetical protein | ||
| uvs040 | Transcription regulator | ||
| uvs044 | Hypothetical protein | ||
| uvs045 | CBS domain protein | ||
| amyA | α-Amylase | ||
| uvs047 | Hypothetical protein | ||
| uvs048 | Glutamyl transpeptidase | ||
| uvs049 | Hypothetical protein | ||
| uvs050 | Transcriptional regulator | ||
| uvs051 | Hypothetical protein | ||
| uvs052 | Hypothetical protein | ||
| uvs053 | Hypothetical protein | ||
| gnuB | Endoglucanase | ||
| exbD | Biopolymer transport protein | ||
| exbB | Biopolymer transport protein | ||
| uvs054 | Hypothetical protein | ||
| uvs055 | Hypothetical protein | ||
| pXIVA3 | uvs061 | Hypothetical protein | |
| uvs062 | GTP binding protein | ||
| uvs063 | GTPase | ||
| uvs064 | Hypothetical protein | ||
| uvs065 | Hypothetical protein | ||
| uvs066 | Hypothetical protein | ||
| uvs067 | Hypothetical protein | ||
| uvs068 | Hypothetical protein | ||
| uvs069 | Hypothetical protein | ||
| actP | Atu037 | ||
| aguC | β-Agarase | ||
| uvs072 | Hypothetical protein | ||
| aguD | β-Agarase I | ||
| aguE | β-Agarase | ||
| uvs075 | Hypothetical protein | ||
| uvs076 | RNA polymerase, σH | ||
| uvs077 | Membrane protein | ||
| uvs078 | Hypothetical protein | ||
| uvs079 | Hypothetical protein | ||
| uvs080 | β-Glucanase | ||
| aguF | MS115 β-agarase | ||
| uvs082 | Regulatory protein | ||
| pXB11 | uvs083 | Hypothetical protein | |
| aguJ | β-Agarase | ||
| aguK | β-Agarase I | ||
| uvs086 | Hypothetical protein | ||
| aguL | β-Agarase | ||
| pIIIE5 | aguG | β-Agarase | |
| aguH | β-Agarase I | ||
| uvs093 | Hypothetical protein | ||
| aguI | β-Agarase | ||
| uvs095 | Hypothetical protein | ||
| uvs96 | Hypothetical protein | ||
| uvs097 | Hypothetical protein | ||
| uvs099 | Hypothetical protein | ||
| uvs100 | Hypothetical protein | ||
| uvs101 | Electron transfer flavoprotein, alpha subunit | ||
| uvs102 | Hypothetical protein | ||
| uvs103 | Hypothetical protein | ||
| uvs104 | Type III secretory protein PscC | ||
| amyB | 1,4-α-Glucan branching enzyme | ||
| uvs098 | Hypothetical protein | ||
| uvs106 | Glucose-1-phosphate adenylyltransferase | ||
| pIIIF2 | uvs110 | Hypothetical protein | |
| uvs111 | Hypothetical protein (possible lipid A biosynthesis protein) | ||
| uvs112 | Hypothetical protein | ||
| Uvs113 | Hypothetical protein | ||
| uvs114 | Hypothetical protein | ||
| glyQ | Glycyl-tRNA synthetase, alpha chain | ||
| glyS | Glycyl-tRNA synthetase, beta chain | ||
| uvs117 | HisB-like phosphatase | ||
| uvs118 | Probable acyltransferase | ||
| uvs119 | Hypothetical protein (possible pectate lyase II) | ||
| pelA | Pectate lyase B | ||
| uvs121 | Hypothetical protein | ||
| uvs122 | Hypothetical protein | ||
| uvs123 | Metallo beta-lactamase | ||
| uvs124 | Monophenol monooxygenase | ||
| uvs125 | Transmembrane protein | ||
| uvs126 | Hypothetical protein | ||
| uvs127 | Hypothetical protein | ||
| uvs128 | 2-Acyl-glycerophospho-ethanolamine acyltransferase |
a
DNA sequences were deposited in the GenBank database under accession numbers AY212801, AY212800, AY236226, AY236224, AY236225, AY240957, AY240958, and AY236223. Identified ORFs were designated by a gene name if the e-value was less than 10−80. Additional data, including the results of similarity searches and the lengths of the predicted ORFs, are available on the web at http://www.gwdg.de/∼biofilm/genemining.htm.