TABLE 1.
Growth and gene expression characteristics of metXW HIRS clones
| B7MX89 clonea | Population size (log CFU/g of leaf)b | Met phenotype in minimal medium | Normalized GFP fluorescencec |
|---|---|---|---|
| pTrap | 3.8 a | − | 16 |
| pMTXW | 5.9 efg | + | 83 |
| pAux.1 | 4.3 b | − | NAd |
| pAux.2 | 4.3 b | − | NA |
| pAux.3 | 4.5 b | − | NA |
| pProt.4 | 5.5 de | + | 46 |
| pProt.5 | 6.6 h | + | 35 |
| pProt.13 | 5.8 ef | + | 157 |
| pProt.14 | 6.0 fg | + | 55 |
| pProt.26 | 5.3 cd | + | 84 |
| pS16.8 | 5.0 c | − | 23 |
| pS16.16 | 5.7 def | − | 27 |
| pS17.3 | 5.6 def | − | 25 |
| pS17.4 | 5.8 ef | − | 28 |
| pS17.15 | 6.4 gh | − | 28 |
a
Bean plants were inoculated with B7MX89 containing pTrap, pMTXW, and pTrap library clones unable (pAux) or able (pProt) to complement methionine auxotrophy of B7MX89 in minimal medium. The pS designation indicates a unique clone enriched by the metXW HIRS system.
b
Data are means for three to five leaves exposed to a 48-h wet-dry cycle. Values with the same letter do not differ significantly by the Fisher's unprotected least-significant-difference test.
c
Average fluorescence value normalized for the cell density (optical density at 600 nm). The coefficient of variation among the GFP fluorescence measurements for each clone was typically 10%.
d
NA, not available.