TABLE 1.
Expression vectors used in experiments
| Vectora | Contents | Source |
|---|---|---|
| pFastBac Dual | polH and p10 promoters | Invitrogen |
| pFBDncp | NCPA in pFastBac Dual (p10 promoter) | This work |
| pFBDncp2A | CYP52A13 in pFBDncp | This work |
| pFBDncp5A | CYP52A17 in pFBDncp | This work |
a
The vector pFBDncp contains NCPA under control of the p10 promoter. The NCPA gene sequence was modified by replacing the single CUG codon (amino acid residue 50) with a UCG encoding serine. Vector pFBDncp2A contains, in addition to NCPA, the gene CYP52A13 under control of the polH promoter. Vector pFBDncp5A contains, in addition to NCPA, CYP52A17 under control of the polH promoter. The two CUG codons present in CYP52A17 (amino acid residues 261 and 490) were also replaced by serine codons UCG and UCU, respectively.