TABLE 3.
NCP activity of microsomes as measured by reduction of cytochrome ca
Vector | NCPA gene | Cytochrome c reduction (μmol/min/mg of protein) |
---|---|---|
pFastBac Dual | − | 0.004 |
pFBDncp2A | + | 0.304 |
pFBDncp5A | + | 0.224 |
Cytochrome P450 reductase activity in insect cell microsomes was determined by reduction of cytochrome c (10). Vector constructs are described in Table 1.