TABLE 5.
Kinetic parameters of the CYP proteins toward myristic and oleic acidsa
| Protein | Substrate | Vmax (nmol/min/ nmol) | Km (μM) | Vmax/Km (min−1 μM−1) |
|---|---|---|---|---|
| CYP52A13 | Myristic acid | 1.27 (0.10) | 110 (34.5) | 0.012 |
| Oleic acid | 1.71 (0.13) | 84.2 (21.7) | 0.020 | |
| CYP52A17 | Myristic acid | 6.36 (0.49) | 23.5 (9.5) | 0.270 |
| Oleic acid | 3.38 (0.43) | 75.4 (29.9) | 0.045 |
a
Conversion rates were measured over 30 min for reactions in which 15% or less of the substrate was converted (linear range) at substrate concentrations of 25, 50, 100, 200, 400, and 800 μM. The values in parentheses are the standard error. The Vmax values were normalized for the amount of cytochrome P450 added based on the concentration estimated by reduced CO difference spectra.