TABLE 1.

Primer and probe sequences used in a multiplex real-time PCR assay to detect stx1, stx2, and the position 93 uidA SNP of E. coli O157:H7/H⁻

Primer or probea	GenBank accession no.	Sequence (5′→3′)	Tm (°C)
stx1F934	M19473	GTGGCATTAATACTGAATTGTCATCA	58
stx1R1042	M19473	GCGTAATCCCACGGACTCTTC	60
stx2F1218	X07865	GATGTTTATGGCGGTTTTATTTGC	60
stx2R1300	X07865	TGGAAAACTCAATTTTACCTTTAGCA	58
uidAF241	AF305917	CAGTCTGGATCGCGAAAACTG	59
uidAR383	AF305917	ACCAGACGTTGCCCACATAATT	59
stx1P990	M19473	Rox-TGATGAGTTTCCTTCTATGTGTCCGGCAGAT-BHQ2	69
stx2P1249	X07865	6FAM-TCTGTTAATGCAATGGCGGCGGATT-BHQ1	69
uidAP266	AF305917	TET-ATTGAGCAGCGTTGG-MGB/NFQ	66

^aPrimer and probe names are composed of the name of the target gene, a letter indicating forward primer (F), reverse primer (R), or probe (P), and the 5′ base position of the oligonucleotide.