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Amplification conditions used for multiplex PCR of stx1, stx2, and the position 93 uidA SNP of E. coli O157:H7/H−
| Parameter seta | MgCl concn (mM) | Annealing and extension temp (°C) | Probe concn (μM)
|
||
|---|---|---|---|---|---|
| stx1 | stx2 | uidA | |||
| A | 3 | 63 | 0.1 | 0.1 | 0.025 |
| B | 4 | 63 | 0.1 | 0.1 | 0.025 |
| C | 2 | 61 | 0.1 | 0.1 | 0.025 |
| D | 2 | 63 | 0.1 | 0.1 | 0.05 |
For the Ct and end fluorescence values obtained with each set of conditions, see Table 3.
