TABLE 4.
Effect of reduced stx2 (FAM) probe concentration on background end fluorescence (dR) in the FAM and TET channelsa
| Isolate | Result in channel with indicated stx2 probe concn (μM)
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
|
stx2 (FAM)
|
uidA SNP (TET)
|
|||||||||
| Genotype | 0.1
|
0.025
|
Genotype | 0.1
|
0.025
|
|||||
| Ct | dR | Ct | dR | Ct | dR | Ct | dR | |||
| 13A71 | Pos. | 19.33 | 315.7 | 19.99 | 104.456 | Neg. | − | 33.897 | − | 28.3 |
| 13A74 | Pos. | 19.93 | 389.5 | 20.36 | 111.5 | Neg. | 24.79b | 44.9 | − | 24.042 |
| 13A75 | Pos. | 19.72 | 409 | 19.68 | 106.828 | Neg. | − | 31 | − | 18 |
| 13A76 | Pos. | 19.7 | 404.422 | 20.09 | 103.389 | Neg. | − | 35.238 | − | 22.571 |
| 13B47 | Pos. | 21.43 | 275.952 | 20.85 | 96.564 | Pos. | 22.91 | 82.714 | 22.31 | 77.714 |
| CDC 3493-88 | Pos. | 20.24 | 268.344 | 19.86 | 102 | Neg. | − | 42 | − | 12.993 |
| 13C06 | Pos. | 19.61 | 306.429 | 19.69 | 106.65 | Neg. | − | 34.571 | − | 33.9 |
| 13C10 | Pos. | 19.34 | 414.762 | 19.83 | 116.75 | Neg. | − | 35.022 | − | 23.25 |
| CFSAN$406 | Pos. | 16.81 | 366 | 17.63 | 104 | Neg. | 21.76b | 51.3 | − | 27 |
| CFSAN$407 | Pos. | 21.08 | 277.571 | 21.12 | 105.5 | Neg. | − | 34.182 | − | 26.3 |
| 3377-85 | Pos. | 19.14 | 344.25 | 19.18 | 114.381 | Neg. | − | 14.1 | − | 24.1 |
| 3493-88 | Pos. | 19.93 | 302.25 | 20.32 | 94.714 | Neg. | − | 23.75 | − | 7.560 |
| 3024-94 | Pos. | 19.76 | 445.133 | 19.93 | 119.286 | Neg. | − | 26 | − | 12.052 |
| EDL 933 | Pos. | 18.54 | 413 | 18.92 | 102.5 | Pos. | 22.91 | 68.091 | 23.24 | 70.286 |
a
Pos., positive; − and Neg., negative.
b
False-positive result due to spectral overlap from the stx2 (FAM) probe at 0.1 μM.