TABLE 5.
Ct values for 138 isolates representing different genotypes for stx1, stx2, and the uidA E. coli O157:H7/H− position 93 SNP
| Organism | Serotype (no. of isolates tested) | Genotypek
|
Avg Ct (SD)
|
||||
|---|---|---|---|---|---|---|---|
| stx1 | stx2 | uidA O157:H7/H− | stx1 | stx2 | uidA O157:H7/H− | ||
| EHEC | O157:H7 (39)a-g | + | + | + | 19.68 (0.548) | 18.98 (0.560) | 23.77 (1.248) |
| O157:H7 (1) | + | − | + | 19.41 | − | 22.21 | |
| O157:H7 (11)a,b,c,e,f | − | + | + | − | 19.71 (1.469) | 23.29 (1.017) | |
| O157:H7 (1) | − | − | + | − | − | 23.30 | |
| O157:H− (1)b | + | + | + | 18.63 | 20.85 | 22.31 | |
| O157:H− (1)f | − | + | + | − | 18.83 | 22.25 | |
| STEC | O68:H− (1)e | + | + | − | 19.43 | 19.11 | − |
| O48: (1) | 18.62 | 19.99 | − | ||||
| O45:H2 (1)g | 19.32 | 19.89 | − | ||||
| O137:H41 (3)g,h | 18.12 (0.125) | 19.96 (0.326) | − | ||||
| O111:H− (3)e,f | 19.47 (0.567) | 19.21 (0.480) | − | ||||
| O22:H8 (1)f | 17.87 | 17.63 | − | ||||
| O15:H27 (1)f | −i | 21.12 | − | ||||
| O4:H− (1)g | 18.92 | 19.18 | − | ||||
| O26:H11 (7)e,f,h | + | − | − | 19.43 (0.757) | − | − | |
| O26:H− (2)e | 20.45 (0.488) | − | − | ||||
| O45:H2 (1)g | 20.18 | − | − | ||||
| O85:H− (1)e | 19.86 | − | − | ||||
| O103:H2 (1)e | 19.82 | − | − | ||||
| O103:H6 (1)e | 19.46 | − | − | ||||
| O111:H11 (1)f | 18.89 | − | − | ||||
| O125:H− (2)f,g | 19.68 (0.092) | − | − | ||||
| O126:H27 (1)g | 18.66 | − | − | ||||
| O146:H21 (2)g | 19.63 (0.269) | − | − | ||||
| Strain with stx1 insert (1)a | 18.99 | − | − | ||||
| O14:H19 (1)g | − | + | − | − | 18.61 | − | |
| O28:H35 (1)h | − | 19.83 | − | ||||
| O48:H21 (1)h | − | 18.40 | − | ||||
| O55:H7 (1)f | − | 21.97 | − | ||||
| O104:H21 (4)d,g | − | 20.02 (0.247) | − | ||||
| O121:H19 (1)g | − | 18.99 | − | ||||
| O165:H25 (3)e | − | 20.00 (0.442) | − | ||||
| Strain with stx2 insert (1)g | − | 19.10 | − | ||||
| Nontoxigenic E. coli | Non-O157:H7 (2)g | − | − | − | − | − | − |
| O55:H7 (1) | − | − | − | ||||
| O157:H16 (3)a | − | − | − | ||||
| O157:H45 (1) | − | − | − | ||||
| Shigella dysenteriae | (1) | + | − | − | 21.39 | − | − |
| Hafnia alvei | (2) | − | − | − | − | − | − |
| Morganella morganii | (1) | − | − | − | − | − | − |
| Citrobacter freundii | (2) | − | − | − | − | − | − |
| Leclercia adecarboxylata | (1) | − | − | − | − | − | − |
| Shigella sonnei | (1)a | − | − | − | − | − | − |
| Shigella boydii | (1)a | − | − | − | − | − | − |
| Shigella flexneri | (1)a | − | − | − | − | − | − |
| Salmonella group 30 | (1) | − | − | − | − | − | − |
| Salmonella enterica serovar Lansing, group P | (1) | − | − | − | − | − | − |
| Klebsiella pneumoniae | (1) | − | − | − | − | − | − |
| Listeria monocytogenes | (1) | − | − | − | − | − | − |
| Listeria innocua | (1) | − | − | − | − | − | − |
| Listeria ivanovii | (1) | − | − | − | − | − | − |
| Listeria seeligeri | (1) | − | − | − | − | − | − |
| Listeria welshimeri | (1) | − | − | − | − | − | − |
| Vibrio cholerae | O1 Inaba (1) | − | − | − | − | − | − |
| Vibrio parahaemolyticus | O4 (1) | − | − | − | − | − | − |
| Vibrio vulnificus | (1) | − | − | − | − | − | − |
| Staphylococcus aureus | (1) | − | − | − | − | − | − |
| Rhodococcus equi | (1) | − | − | − | − | − | − |
| Lactobacillus sp. | (2) | − | − | − | − | − | −/PICK> |
| Salmonella enterica serovar Typhimurium | (1)a | − | − | − | − | − | − |
| Streptococcus pyogenes | (1) | − | − | − | − | − | − |
| Alcaligenes faecalis | (1) | − | − | − | − | − | − |
| Salmonella enterica serovar Choleraesuis | (1) | − | − | − | − | − | − |
| Yersinia enterocolitica | (2) | − | − | − | − | − | − |
| Enterobacter cloacae | (1) | − | − | − | − | − | − |
| Avgj | 19.51 (0.713) | 19.32 (0.906) | 23.56 (1.225) | ||||
Isolate received from the Washington State Department of Health.
Isolate received from the Oregon State Department of Health.
Isolate received from the King County Public Health Department.
Isolate received from the Montana Public Health Laboratory.
Isolate received from Phil Tarr, Children's Hospital Seattle, Wash.
Isolate received from Peter Feng, Center for Food Safety and Nutrition, Food and Drug Administration.
Isolate received from Joy Wells, Centers for Disease Control and Prevention.
Isolate received from Sharon Abbott, California Health Department.
False-negative result obtained under these reaction conditions.
Overall average Ct for positive isolates (n = 74, 76, and 54 for stx1, stx2, and uidA, respectively).
+, positive; −, negative.