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. 2003 Oct;69(10):6294–6298. doi: 10.1128/AEM.69.10.6294-6298.2003

FIG. 2.

FIG. 2.

FISH on dental plaque samples from human subgingival sites of moderate chronic periodontitis (A to E) and from healthy sites (F). (A.1 to A.3) Staining with YO-PRO-1 (green) general stain (A.1) and TM7-905-Cy5 (blue) (A.2) and colocalization of these two stains (A.3). Asterisks, two segments of the TM7 filament that are not labeled by the probe. In all other panels, YO-PRO-1 and probe signals are merged as in panel A.3. (B and C) TM7-905 probe and YO-PRO-1-labeled cells (blue-green); (D to F) TM7-905 probe-labeled cells (solid arrowhead, dark blue) plus IO25-136 probe-labeled cells (open arrowhead, light blue) and cells labeled with YO-PRO-1 alone (green). Scale bar = 5 μm in all panels.