TABLE 1.
Recombinant 16S rDNA plasmids and strains used in this study
| Probe, plasmid, or strain | Sequenceg (5′-3′) |
|---|---|
| TM7-905 | CCGTCAATTCCTTTATGTTTTA |
| pSBG1a | ---------------------- |
| pSBG1ab | ---------G------------ |
| pSBG1bb | ---------------------- |
| pSBG2a | ---------------------- |
| pMF1c | ---------------A------ |
| A. aquaticusd | --------------GA------ |
| pPEe | --A-----------GA----C- |
| IO25-136 | GTCTTATCCCTCACTGCAGG |
| pSBG1 | -------------------- |
| pSBG1a | -------------------- |
| pSBG1b | -----------G-------- |
| pSBG2 | --CAA-------------C- |
| pMF1 | TCGC------AAT---AGA-f |
| A. aquaticus | AAA----T--GT--GATT--f |
| pPE | -GT-AT-----A-T-A----f |
Created through site-directed mutagenesis of pSBG1.
Created from M. felifaucium ATCC 43428.
ATCC 25396.
Created from P. endodontalis ATCC 35406.
Source: Ribosomal Database Project (9).
The sequences of oligonucleotide probes TM7-905 and IO25-136 are compared with the corresponding sequences at the probe hybridization sites in the plasmids and strain. Only the nucleotides that differ from those of the probe sequences are shown.