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. 2003 Oct;69(10):6294–6298. doi: 10.1128/AEM.69.10.6294-6298.2003

TABLE 1.

Recombinant 16S rDNA plasmids and strains used in this study

Probe, plasmid, or strain Sequenceg (5′-3′)
TM7-905 CCGTCAATTCCTTTATGTTTTA
    pSBG1a ----------------------
    pSBG1ab ---------G------------
    pSBG1bb ----------------------
    pSBG2a ----------------------
    pMF1c ---------------A------
    A. aquaticusd --------------GA------
    pPEe --A-----------GA----C-
IO25-136 GTCTTATCCCTCACTGCAGG
    pSBG1 --------------------
    pSBG1a --------------------
    pSBG1b -----------G--------
    pSBG2 --CAA-------------C-
    pMF1 TCGC------AAT---AGA-f
    A. aquaticus AAA----T--GT--GATT--f
    pPE -GT-AT-----A-T-A----f
a

Accession numbers AY144353 (pSBG1) and AY144354 (pSBG2).

b

Created through site-directed mutagenesis of pSBG1.

c

Created from M. felifaucium ATCC 43428.

d

ATCC 25396.

e

Created from P. endodontalis ATCC 35406.

f

Source: Ribosomal Database Project (9).

g

The sequences of oligonucleotide probes TM7-905 and IO25-136 are compared with the corresponding sequences at the probe hybridization sites in the plasmids and strain. Only the nucleotides that differ from those of the probe sequences are shown.