TABLE 2.
Characteristics of predicted proteins specified by the C. beijerinckii ATCC 25752 cir gene cluster
| Protein | Gene sizea (bp) | Protein
|
||||
|---|---|---|---|---|---|---|
| Size (kDa) | pl | TMb | Homologyc | Putative function | ||
| CfgR | 774 | 30.4 | 6 | 0 | Response regulators | |
| CfgK | 1,275 | 49.8 | 6.8 | 7 | Histidine kinases | |
| Cfg01 | 597 | 23.2 | 9.8 | 5 | AgrB regulatory protein | |
| Cfg02 | 390 | 14.4 | 10.17 | 4 | Unknown | |
| CirA | 216 | 7.2 | 10.9d | 2 | Enterocin AS-48 | CirA precursor |
| CirB | 1,743 | 68.8 | 9.4 | 11 | AS-48B (19%) | Secretion/immunity |
| CirC | 555 | 20.9 | 10.1 | 4 | AS-48C (21%) | Maturation |
| CirD | 663 | 25.7 | 6.4 | 0 | ATP-binding proteins; AS-48D (31.6%) | Secretion/immunity |
| CirE | 147 | 5.7 | 10.6 | 2 | AS-48D1 (30%) | Immunity |
| CirG | 1,425 | 51.7 | 4.6 | 1 | HlyD family of proteins/EmrA/BacG (17%) | |
| CirH | 744 | 27.6 | 6.1 | 0 | ATP-binding proteins/BacH (40%) | |
| CirI | 1,266 | 45.7 | 9.7 | 4 | Duf214 domain/permease of ABC transporter/Bacl (32%) | |
a
When multiple putative starts were possible, the longest product was used.
b
TM, number of putative transmembrane sequences.
c
Homologues were identified by using Blast searches against the NCBI nonredundant protein database or by direct comparison to the proteins involved in enterocin AS-48 production. The identity of the protein to its homologue involved in enterocin AS-48 production is shown in parentheses.
d
The pl of CirA is based on the linear unprocessed protein.