FIG. 1.
Proteolytic activity of L. rhamnosus BGT10. (A) Caseins hydrolyzed by whole cells collected from MCA plates. Lanes: α, αS1-casein substrate; 1, hydrolyzed αS1-casein; β, β-casein substrate; 2, hydrolyzed β-casein. (B) Nutrient-dependent proteolytic activity of L. rhamnosus BGT10. Lanes: β, β-casein substrate; 1 to 4, β-casein hydrolyzed by whole cells collected from CDM plates containing 0.1, 0.5, 1, and 2% Casitone, respectively; 5, whole cells collected from MRS plates; 6, whole cells collected from MCA plates. (C) Hydrolysis of β-casein by the proteinase extracts. Lanes: β, β-casein substrate; 1, β-casein hydrolyzed by extract obtained from cells grown on CDM plates containing 0.1% Casitone; 2, β-casein hydrolyzed by extract obtained from the cells grown on MCA plates. Arrows indicate α- or β-casein.