TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
| Strains | ||
| L. rhamnosus | ||
| BGT10 | Human vaginal isolate | This study |
| BGT16 | Human vaginal isolate | This study |
| BGT30 | Human vaginal isolate | This study |
| BGT32 | Human vaginal isolate | This study |
| ATCC 7469 | Type strain | American Type Culture Collection |
| BGTM1 | Natural isolate from soft homemade cheese | 53 |
| BGEN1 | Natural isolate from soft homemade cheese | 27 |
| BGZL18 | Natural isolate from soft homemade cheese | This study |
| BGZL20 | Natural isolate from soft homemade cheese | This study |
| CRL576 | Type strain | CERELA Collection |
| CRL932 | Type strain | CERELA Collection |
| CRL1036 | Type strain | CERELA Collection |
| CRL1227 | Type strain | CERELA Collection |
| L. lactis MG1363 | Lac− Prt−; plasmid-free derivative of NCDO 712 | 14 |
| L. casei ATCC 393T | pLZ15− Lac− | 5 |
| E. coli DH5α | F− φ80dlacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17 (rK− mK+) supE44 λ−thi-1 gyrA96 relA1 | Laboratory collection |
| Plasmids | ||
| pGEM-T Easy | Ampr, M13ori pBR322ori, linear T-overhang vector | Promega |
| pUC18 | Ampr | 54 |
| pXS200 | Ampr, pUC18 carrying 2-kb XbaI-SphI chromosomal fragment from 3′ part of prtR gene | This study |
| pNZ273 | Cmr, promoter probe vector containing promoterless gusA gene | 43 |
| pEB640 | pNZ273 carrying T10Bam-T10Eco PCR fragment in front of the gusA gene | This study |
| pEB260 | pNZ273 carrying TR1035-T10Eco PCR fragment in front of the gusA gene | This study |
| pEB122 | pNZ273 carrying TR180-T10Eco PCR fragment in front of the gusA gene | This study |
| pEB471 | pNZ273 carrying T10Bam-TR237 PCR fragment in front of the gusA gene | This study |
| pEB99 | pNZ273 carrying TR1035-TR237 PCR fragment in front of the gusA gene | This study |