TABLE 2.
Expression of the prtR-gusA fusions in CDM supplemented with casitone and in MRS broth
| Transformant | β-Glucuronidase activity (mean ± SD)a in:
|
Induction ratio (0.1%/2% Casitone) | ||||
|---|---|---|---|---|---|---|
| CDM supplemented with Casitone at:
|
MRS | |||||
| 0.1% | 0.5% | 1% | 2% | |||
| ATCC 393T/pEB640 | 93 ± 44 | 31 ± 23 | 8 ± 6 | 10 ± 5 | 3 ± 2 | 9.30 |
| ATCC 393T/pEB260 | 181 ± 86 | 50 ± 13 | 20 ± 8 | 14 ± 5 | 3 ± 1 | 12.93 |
| ATCC 393T/pEB471 | 121 ± 51 | 54 ± 24 | 45 ± 34 | 20 ± 13 | 15 ± 7 | 6.05 |
| ATCC 393T/pEB99 | 337 ± 167 | 129 ± 57 | 75 ± 10 | 50 ± 14 | 12 ± 2 | 6.74 |
| ATCC 393T/pEB122 | 0 | 0 | 0 | 0 | 0 | |
a
Expressed as nanomoles per minute per milligram of protein. Values are means and standard deviations from three independent determinations.