TABLE 1.
EaCoMT activity in cell extracts of alkene-assimilating Mycobacterium strains
| Strain | Isolation and growth substrate | EaCoMT activitya (nmol/min/mg of protein)
|
|
|---|---|---|---|
| CoM added | No cofactor | ||
| JS60 | VC | 980 (930-1,060)b | 0 (0)b |
| JS61 | VC | 2,910 (2,330-3,490) | 110 (70-160) |
| JS616 | VC | 1,800 (1,710-1,890) | 10 (0-20) |
| JS617 | VC | 900 (710-1,090) | 30 (20-40) |
| JS619 | VC | 1,920 (1,880-1,960) | 130 (130) |
| JS621 | VC | 790 (780-800) | 110 (110) |
| JS622 | Ethene | 930 (850-1,000) | 50 (0-100) |
| JS623 | Ethene | 590 (400-780) | 60 (0-120) |
| JS624 | Ethene | 470 (440-500) | 10 (0-20) |
| JS625 | Ethene | 380 (290-460) | 0 (0) |
a
The results are averages of two assays, and the ranges are in parentheses. Reaction mixtures (1 ml) contained 0.1 mg of protein, 5 μmol of epoxyethane, and 10 μmol of CoM. Specific activities were calculated from the epoxyethane depletion rate after subtraction of the appropriate abiotic rate of epoxyethane loss (either 2.5 nmol/min in Tris buffer or 13 nmol/min in Tris buffer plus CoM).
b
From a previous study (5a).