TABLE 1.
Nucleotide sequences and positions of primers used in this study
| Primera | Sequenceb | Position | GenBank accession no. |
|---|---|---|---|
| GFP1 | 5′-TAGCAGATCTAAAATGAGTAAAGGAGAAGAAC-3′ | 286-307 | U62636 |
| GFP2 | 5′-TCGGAGATCTGTTGGAATTCATTATTTGTAGAG-3′ | 994-1026 | U62636 |
| SB2 | 5′-GTGAAGCACCAGTTTCTGAACC-3′ | 4184-4205 | AF012877 |
| SR5 | 5′-GGTCAGATCTAGCCAGAATTAAAAGTTAGTG-3′ | 4141-4171 | AF012877 |
| SR8 | 5′-AAGTTCGGATCCTGCTTTTGGTGGTGC-3′ | 5184-5210 | AF012877 |
| SR12 | 5′-GTGTATCAGCTGTCGGAAC-3′ | 4513-4531 | AF012877 |
| SR14 | 5′-TGTCGGATCCACATCAGTGGTTGCATGTAAT-3′ | 4523-4553 | AF012877 |
| SR16 | 5′-CTTTTTGATCTGAATAGTTTCCCGCATCAC-3′ | 5068-5097 | AF012877 |
| SR17 | 5′-TGTCGGAACAAGATCTGTGGTTGCATGTAAT-3′ | 4523-4553 | AF012877 |
| SR23 | 5′-CTTGTGAATCACCACCAGATG-3′ | 5380-5400 | AF012877 |
| SR24 | 5′-GCAAGTCAAGCGGGAC-3′ | 4069-4084 | AF012877 |
| TET8 | 5′-GGAGAAATCCCTGCTCGGTG-3′ | 1957-1976 | X56353 |
| CJ6 | 5′-CAATTACCAACCATGTTAGC-3′ | 3576-3595 | U89875 |
| CJ8 | 5′-GGTTAGTAATGCTGATCGC-3′ | 2328-2346 | U89875 |
| U | 5′-GTAAAACGACGGCCAGT-3′ | 379-395 | L09137 |
| R | 5′-GGAAACAGCTATGACCATG-3′ | 461-479 | L09137 |
a
Primers GFPn, SB2 and SRn, Tet8, CJn, and U and R refer to GFP, the spiralin gene of S. citri, the tetM gene of Tn916, the scm1 gene of S. citri, and the universal and reverse primers of pUC19, respectively.
b
Restriction sites used for cloning are underlined.