Figure 2.

Identification of iNOS mRNA-positive mononuclear cells in the lumen of a blood vessel in the vicinity of an MS plaque (A) and the association of iNOS mRNA-positive cells with accumulations of nitrotyrosine in an MS plaque (B). (A) A brain section containing an MS plaque was stained with antibody to vWF (yellow/orange) and with RT-IS-PCR using a fluorescein isothiocyanate-labeled probe specific for iNOS mRNA (green). (B) A section of an MS plaque was stained for iNOS with RT-IS-PCR (green) and antibodies to nitrotyrosine. The sections were prepared, stained, and visualized as described in Materials and Methods and the legend to Fig. 1. The image in A was photographically enlarged approximately ×10.