TABLE 3.
Substrate specificities of rMCO1, laccase from C. subvermispora, and laccase 51002
| Compound | Oxygen consumption (nmol/min)a
|
||
|---|---|---|---|
| rMCO1 | Laccase 51002 | rLcs1 | |
| Fe2+ | 39 | 8.6 | 4.6 |
| o-Dianisidine | 23 | 35 | 28 |
| p-Anisidine | 9.0 | 5.1 | 9.2 |
| 1,4-Phenyldiamine | 52 | 37 | 23 |
| 2,6-Dimethoxyphenol | 5.1 | 52 | 23 |
| Syringaldazine | 0 | 28 | 12 |
| ABTS | 3.2 | 52 | 21 |
| Guaiacol | 1.8 | 44 | 17 |
| Gallic acid | 4.6 | 32 | 7.4 |
| Phloroglucinol | 0 | 15 | 1.9 |
| Pyrogallol | 5.5 | 52 | 12 |
| Resorcinol | 1.1 | 4.4 | 1.8 |
a
Averages for triplicate assays performed with 1 μg of enzyme in 0.1 mM acetate buffer (pH 5.0). In most cases the substrate concentration was 1 mM; the concentration of syringaldazine used was 0.1 mM.