TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Reference or source |
|---|---|---|
| E. coli strains | ||
| XL1-Blue | supE44 hsdR17 recA1 endA1 gyrA96 thi relA1 | Stratagenea |
| BL21 (DE3) | lac F′[proAB+laclqlacZΔM15 Tn10(Tetr)] F−ompT hsdSB (rB− mB−) gal dcm (DE3) | Novagenb |
| Plasmids | ||
| p10499A | 4.20 kb, Apr, gntT104promoter | Lab stock |
| p104CysK | 5.20 kb, Apr, gntT104promoter | This study |
| pACYC184 | 4.30 kb, Cmr, Tcr | New England Biolabsc |
| pAC104CysK | 6.20 kb, Cmr Tcr, gntT104promoter | This study |
| pEDCSFmII | 5.90 kb, human G-CSF gene, T7 promoter | 18 |
| pEDOb5 | 5.75 kb, Apr, human obese gene, T7 promoter | 17 |
| pUC18/p40 | 3.67 kb, Apr, human IL-12p40 gene | BCRd |
| pEDIL-12p40 | 6.1 kb, Apr, human IL-12p40 gene, T7 promoter | This study |
a
Stratagene Cloning Systems, La Jolla, Calif.
b
Novagen, Inc., Madison, Wis.
c
New England Biolabs, Inc., Beverly, Mass.
d
BCR, Bank for Cytokine Research, Chonbuk National University, Jeollabuk-do, Korea.