TABLE 2.
Cross-reactivity in reverse line blot hybridization of probes to control templates with few mismatches
| Probe and clone | Target sequence | Cross- reactivityb |
|---|---|---|
| Probe Rho-BAL47-396 | CATTCCGCGTGCAGGA | |
| Clone URK0-13 | ---G------------ | − |
| Clone Sta5-10 | ---G------------ | − |
| Clone LD2 | ---C------------ | − |
| Clone Med0-02 | ---C------------ | − |
| Clone LD3 | ---G--A--------- | − |
| Clone LD21 | ---C-------A---- | − |
| Clone LD1 | ---C-------G---- | − |
| Clone URK0-17 | ---G--------G--- | − |
| Probe Rpickettii-490 | GGAAGAATAAGGACCGGCT | |
| Clone LD17 | T----------C------- | − |
| Clone LD3 | T------------T----- | − |
| Clone URK0-13 | -T-----------T----- | − |
| Probe CR-FL23-464a | TAACACAG•CTGTGAAUGACGG | |
| Clone LD17 | --------T------------- | − |
| Probe Sta230-187 | GCATCACTGAGTTTTGAAAGAATT | |
| Clone Sta5-26 | -----G------------------ | + |
| Probe Urk014-126 | GAGAAACCAACCCCGGTC | |
| Clone Sta2-06 | --------TG---T---- | − |
| Probe Syn/Pro-415 | AGGCCTCTGGGCTGTAAAC | |
| Clone LD6 | ------G----T------- | − |
| Clone URK0-03 | ------TC----------- | − |
| Clone MED0-06 | ------TC----------- | − |
a
Probe CR-FL23-464 and clone LD17 differ by an insertion or deletion as indicated by the bullet.
b
That is, the presence (+) or absence (−) of reactivity of probes to the mismatching templates (0.2 pmol of PCR product [F27-R518] per slot).