TABLE 1.
HPS and PHI activities in cell extracts of B. cepacia TM1 grown on media with various carbon sourcesa
| Medium and carbon source (concn) | Enzyme activity (U/min/mg of protein)
|
|
|---|---|---|
| HPS | PHI | |
| LB medium | <0.01 | <0.01 |
| LB medium + formaldehyde (0.7 mM) | 0.37 | 0.21 |
| Mineral salt medium + gluconic acid (45 mM) | <0.01 | 0.01 |
| Mineral salt medium + vanillin (15 mM) | 0.30 | 0.20 |
| Mineral salt medium + vanillic acid (15 mM) | 0.28 | 0.15 |
| Mineral salt medium + protocatechuic acid (15 mM) | 0.05 | 0.04 |
The assays were done in duplicate, and each reading did not vary from the mean by more than 30%.