Table 1.
α-Gal A activity of fibroblast clones transduced with bicistronic MDR retroviruses containing human α-Gal A cDNA
| Clone | Genotype | Retrovirus | α-Gal A activity,* nmol/h per mg | Lectin (GSL-B4) staining† |
|---|---|---|---|---|
| N1 | N | — | 527.9 | − |
| N2 | N | — | 636.0 | − |
| K1 | KO | — | 1.4 | + |
| K2 | KO | — | 1.7 | + |
| KM-1 | KO | HaMDR | 38.8 | + |
| KM-2 | KO | HaMDR | 4.2 | + |
| KGM-1 | KO | Ha-αGal-IRES-MDR | 611.0 | − |
| KMG-1 | KO | Ha-MDR-IRES-αGal | 524.8 | − |
| KMG-2 | KO | Ha-MDR-IRES-αGal | 274.8 | − |
Primary fibroblasts from mice of either genotypes were established using 14.5–16.5 E embryos as described (22). Cultured fibroblasts were transduced with indicated retroviruses as described (11). N, normal; KO, knockout.
*
Data are expressed as means of three determinations.
†
Fluorescence-labeled lectin binding method as shown in Fig. 4.