FIG. 1.

Specific binding saturation of ¹²⁵I-Cry1Aa (A), ¹²⁵I-Cry1Ab (B), ¹²⁵I-Cry1Ac (C), and ¹²⁵I-Cry2Aa (D) to BBMV proteins from YDK (•), CXC (▿) or KCBhyb (▪) larvae. BBMV proteins (10 μg) were incubated with increasing amounts of labeled toxins for 1 h. Nonspecific binding was calculated in the presence of 1,000 nM of the respective unlabeled homologous toxin and was subtracted from total binding to obtain specific binding. Binding reactions were stopped by centrifugation. Bound labeled toxin (nanomolar concentration) was calculated using the RADLIG software. Each data point is a mean based on at least two experiments done in quadruplicate. Error bars depict standard deviation of the mean values.