FIG. 3.

Irreversible binding of ¹²⁵I-Cry1Ac toxin to BBMV from YDK (•), CXC (▿), or KCBhyb (▪) larvae. Binding reactions were started by mixing BBMV proteins (10 μg) with 0.1 nM ¹²⁵I-Cry1Ac. One hour after initiation of the binding reaction, 1,000 nM unlabeled Cry1Ac was added to the mixture. The time on the x axis represents postincubation time after addition of the unlabeled competitor. Binding was expressed as a percentage of the amount of toxin bound before addition of competitor. Each data point is a mean based on data from two independent trials done in dupliacte. Error bars depict standard deviation of the mean values.