TABLE 5.
TNE activitya
| Strain | Expression construct | No. of Hygr colonies | Correction efficiency (105) |
|---|---|---|---|
| LSY678(Int)Hyg(rep) | 1,290 ± 119 | 3.21 ± 0.29 | |
| LSY678(Int)Hyg(rep) | pSM258:mre11(wt) | 3,796 ± 318 | 9.58 ± 0.48 |
| LSY678(Int)Hyg(rep) | pSM304:mre11-H125N | 2,072 ± 219 | 3.52 ± 0.53 |
| LSY678(Int)Hyg(rep) | pSM312:mre11-D56N | 1,668 ± 117 | 4.40 ± 0.97 |
| LSY678(Int)Hyg(ins) | 716 ± 64 | 2.56 ± 0.28 | |
| LSY678(Int)Hyg(ins) | pSM258:mre11(wt) | 992 ± 109 | 6.16 ± 0.68 |
| LSY678(Int)Hyg(ins) | pSM304:mre11-H125N | 556.17 ± 72.28 | 3.52 ± 0.49 |
| LSY678(Int)Hyg(ins) | pSM312:mre11-D56N | 492.28 ± 59.04 | 3.13 ± 0.45 |
a
LSY678(Int)Hyg(rep) and LSY678(Int)Hyg(ins) containing episomal plasmid pSM258:mre11(wt), pSM304:mre11-H125N, or pSM312:mre11-D56N were targeted with 5 μg of Hyg3S/74NT, and then the correction efficiency was assessed by determining colony growth on YPD plates containing hygromycin, as described in Materials and Methods. The values are averages ± standard deviations for three experiments.