FIG. 2.
PAGE and determination of the molecular mass of XR purified from C. parapsilosis. Shown are results from native PAGE (A) and SDS-PAGE (B). The enzyme solution was run on a 10% (wt/vol) polyacrylamide slab gel as described in Materials and Methods. The arrow indicates the protein band containing XR. (C) Determination of the molecular mass of native C. parapsilosis XR by gel filtration chromatography. The chromatography runs were performed as described in Materials and Methods. The marker proteins used and their Mrs were as follows: β-amylase, 200 kDa; alcohol dehydrogenase, 150 kDa; bovine serum albumin, 66 kDa; carbonic anhydrase, 29 kDa; cytochrome c, 12.4 kDa. The arrow indicates the position for the XR molecular mass from C. parapsilosis. Kav = (Ve − Vo)/(Vt − Vo) where Ve is the elution volume of protein, Vo is the elution volume of Blue Dextran 2000, and Vt is total bed volume.