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Effects of substrate concentration on the activities of XR. XR activity of the enzyme (1 U) was measured in the presence of the indicated concentrations of d-xylose and 0.25 mM NADH at pH 6.0. The inset shows Lineweaver-Burk plot of initial velocity versus various fixed d-xylose concentrations. Each value represents the mean of triplicate measurements and varied from the mean by not more than 10%.
