FIG. 5.
Graphical analysis of the inhibition of C. parapsilosis XR by xylitol. The effects of increasing xylitol (product) concentration on the apparent Km and Vmax values for d-xylose and NADH were examined. Analysis of these data by double-reciprocal plots indicated that xylitol inhibited XR noncompetitively with respect to d-xylose (A) and NADH (B). Also shown are the secondary plots for noncompetitive inhibition with d-xylose and NADH (C). The xylitol product binds to XR with a Ki of 368 mM.